CLIA Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

CLIA Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

Marca: Cloud Clone

Catálogo: SCB932Hu

Faixa de Detecção: 0.27-200ng/mL

Aplicação: ELISA

Espécie: Homo sapiens (Human)

Sensibilidade: The minimum detectable dose of this kit is typically less than 0.12ng/mL

Duração do ensaio: 2h, 40min

Método: Double-antibody Sandwich

Descrição Protocolo: The microplate provided in this kit has been pre-coated with an antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon