CLIA Kit for Pigment Epithelium Derived Factor (PEDF)

CLIA Kit for Pigment Epithelium Derived Factor (PEDF)

Marca: Cloud Clone

Catálogo: SCB972Hu

Faixa de Detecção: 0.16-120ng/mL

Aplicação: ELISA

Espécie: Homo sapiens (Human)

Sensibilidade: The minimum detectable dose of this kit is typically less than 0.07ng/mL

Duração do ensaio: 2h, 40min

Método: Double-antibody Sandwich

Descrição Protocolo: The microplate provided in this kit has been pre-coated with an antibody specific to Pigment Epithelium Derived Factor (PEDF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Pigment Epithelium Derived Factor (PEDF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate developme