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EntendiELISA Kit for 2-Arachidonoylglycerol (2-AG)
Marca: Cloud Clone
Catálogo: CEO443Ge
Faixa de Detecção: 3.70-300ng/mL
Aplicação: ELISA
Espécie: Pan-species (General)
Sensibilidade: The minimum detectable dose of this kit is typically less than 1.44ng/mL
Duração do ensaio: 2h
Método: Competitive Inhibition
Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 2-AG has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 2-AG analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conju