ELISA Kit for 25-Hydroxyvitamin D3 (HVD3)

ELISA Kit for 25-Hydroxyvitamin D3 (HVD3)

Marca: Cloud Clone

Catálogo: CEA915Ge

Faixa de Detecção: 2.47-200ng/mL

Aplicação: ELISA

Espécie: Pan-species (General)

Sensibilidade: The minimum detectable dose of this kit is typically less than 0.91ng/mL

Duração do ensaio: 2h

Método: Competitive Inhibition

Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 25-Hydroxyvitamin D3 (HVD3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 25-Hydroxyvitamin D3 (HVD3) and unlabeled 25-Hydroxyvitamin D3 (HVD3) (Standards or samples) with the pre-coated antibody specific to 25-Hydroxyvitamin D3 (HVD3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradis