ELISA Kit for Anandamide (AEA)

ELISA Kit for Anandamide (AEA)

Marca: Cloud Clone

Catálogo: CEO440Ge

Faixa de Detecção: 2.47-200ng/mL

Aplicação: ELISA

Espécie: Pan-species (General)

Sensibilidade: The minimum detectable dose of this kit is typically less than 0.93ng/mL

Duração do ensaio: 2h

Método: Competitive Inhibition

Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to anandamide has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled anandamide analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bou