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EntendiELISA Kit for Apelin (APLN)
Marca: Cloud Clone
Catálogo: CED066Hu
Faixa de Detecção: 19.75-1,600pg/mL
Aplicação: ELISA
Espécie: Homo sapiens (Human)
Sensibilidade: The minimum detectable dose of this kit is typically less than 8.25pg/mL
Duração do ensaio: 2h
Método: Competitive Inhibition
Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Apelin (APLN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Apelin (APLN) and unlabeled Apelin (APLN) (Standards or samples) with the pre-coated antibody specific to Apelin (APLN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and