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EntendiELISA Kit for Beta-Endorphin (bEP)
Marca: Cloud Clone
Catálogo: CEA806Ra
Faixa de Detecção: 12.35-1,000pg/mL
Aplicação: ELISA
Espécie: Rattus norvegicus (Rat)
Sensibilidade: The minimum detectable dose of this kit is typically less than 4.53pg/mL
Duração do ensaio: 2h
Método: Competitive Inhibition
Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-Endorphin (bEP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-Endorphin (bEP) and unlabeled Beta-Endorphin (bEP) (Standards or samples) with the pre-coated antibody specific to Beta-Endorphin (bEP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added