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EntendiELISA Kit for Diacylglycerol (DAG)
Marca: Cloud Clone
Catálogo: CEC038Ge
Faixa de Detecção: 391-100,000pg/mL
Aplicação: ELISA
Espécie: Pan-species (General)
Sensibilidade: The minimum detectable dose of this kit is typically less than 153pg/mL
Duração do ensaio: 2h
Método: Competitive Inhibition
Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Diacylglycerol (DAG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Diacylglycerol (DAG) and unlabeled Diacylglycerol (DAG) (Standards or samples) with the pre-coated antibody specific to Diacylglycerol (DAG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added