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EntendiELISA Kit for Endothelin 2 (EDN2)
Marca: Cloud Clone
Catálogo: CEF415Ra
Faixa de Detecção: 6.17-500pg/mL
Aplicação: ELISA
Espécie: Rattus norvegicus (Rat)
Sensibilidade: The minimum detectable dose of this kit is typically less than 2.67pg/mL
Duração do ensaio: 2h
Método: Competitive Inhibition
Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Endothelin 2 (EDN2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Endothelin 2 (EDN2) and unlabeled Endothelin 2 (EDN2) (Standards or samples) with the pre-coated antibody specific to Endothelin 2 (EDN2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to e