ELISA Kit for Free Thyroxine (fT4)

ELISA Kit for Free Thyroxine (fT4)

Marca: Cloud Clone

Catálogo: CEA185Ge

Faixa de Detecção: 1.11-90pg/mL

Aplicação: ELISA

Espécie: Pan-species (General)

Sensibilidade: The minimum detectable dose of this kit is typically less than 0.42pg/mL

Duração do ensaio: 2h

Método: Competitive Inhibition

Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to T4 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled T4 analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate