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EntendiELISA Kit for Insulin (INS)
Marca: Cloud Clone
Catálogo: CEA448Gu
Faixa de Detecção: 123.5-10,000pg/mL
Aplicação: ELISA
Espécie: Cavia (Guinea pig )
Sensibilidade: The minimum detectable dose of this kit is typically less than 51.5pg/mL
Duração do ensaio: 2h
Método: Competitive Inhibition
Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Insulin (INS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Insulin (INS) and unlabeled Insulin (INS) (Standards or samples) with the pre-coated antibody specific to Insulin (INS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and