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EntendiELISA Kit for Nesfatin 1 (NES1)
Marca: Cloud Clone
Catálogo: CEA242Mu
Faixa de Detecção: 493.8-40,000pg/mL
Aplicação: ELISA
Espécie: Mus musculus (Mouse)
Sensibilidade: The minimum detectable dose of this kit is typically less than 183.4pg/mL
Duração do ensaio: 2h
Método: Competitive Inhibition
Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Nesfatin 1 (NES1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Nesfatin 1 (NES1) and unlabeled Nesfatin 1 (NES1) (Standards or samples) with the pre-coated antibody specific to Nesfatin 1 (NES1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each micr