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EntendiELISA Kit for Neuropeptide FF (NPFF)
Marca: Cloud Clone
Catálogo: CEG003Ra
Faixa de Detecção: 12.5-200pg/mL
Aplicação: ELISA
Espécie: Rattus norvegicus (Rat)
Sensibilidade: The minimum detectable dose of this kit is typically less than 4.7pg/mL
Duração do ensaio: 2h
Método: Competitive Inhibition
Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neuropeptide FF (NPFF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neuropeptide FF (NPFF) and unlabeled Neuropeptide FF (NPFF) (Standards or samples) with the pre-coated antibody specific to Neuropeptide FF (NPFF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) i