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EntendiELISA Kit for Ouabain (OB)
Marca: Cloud Clone
Catálogo: CEV857Ge
Faixa de Detecção: 617.3-50,000pg/mL
Aplicação: ELISA
Espécie: Pan-species (General)
Sensibilidade: The minimum detectable dose of this kit is typically less than 252.4pg/mL
Duração do ensaio: 2h
Método: Competitive Inhibition
Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ouabain (OB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ouabain (OB) and unlabeled Ouabain (OB) (Standards or samples) with the pre-coated antibody specific to Ouabain (OB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incu