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EntendiELISA Kit for Pepsin (PP)
Marca: Cloud Clone
Catálogo: CEA632Ra
Faixa de Detecção: 2.47-200ng/mL
Aplicação: ELISA
Espécie: Rattus norvegicus (Rat)
Sensibilidade: The minimum detectable dose of this kit is typically less than 0.90ng/mL
Duração do ensaio: 2h
Método: Competitive Inhibition
Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Pepsin (PP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Pepsin (PP) and unlabeled Pepsin (PP) (Standards or samples) with the pre-coated antibody specific to Pepsin (PP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubate