ELISA Kit for Prothrombin Fragment 1+2 (F1+2)

ELISA Kit for Prothrombin Fragment 1+2 (F1+2)

Marca: Cloud Clone

Catálogo: SEA710Mu

Faixa de Detecção: 0.312-20ng/mL

Aplicação: ELISA

Espécie: Mus musculus (Mouse)

Sensibilidade: The minimum detectable dose of this kit is typically less than 0.114ng/mL

Duração do ensaio: 3h

Método: Double-antibody Sandwich

Descrição Protocolo: The microplate provided in this kit has been pre-coated with an antibody specific to F1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate r