ELISA Kit for Vitamin B1 (VB1)

ELISA Kit for Vitamin B1 (VB1)

Marca: Cloud Clone

Catálogo: CED053Ge

Faixa de Detecção: 3.70-300ng/mL

Aplicação: ELISA

Espécie: Pan-species (General)

Sensibilidade: The minimum detectable dose of this kit is typically less than 1.63ng/mL

Duração do ensaio: 2h

Método: Competitive Inhibition

Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vitamin B1 (VB1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vitamin B1 (VB1) and unlabeled Vitamin B1 (VB1) (Standards or samples) with the pre-coated antibody specific to Vitamin B1 (VB1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each micropla