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EntendiELISA Kit for Vitamin B7 (VB7)
Marca: Cloud Clone
Catálogo: CEG162Ge
Faixa de Detecção: 32-20,000pg/mL
Aplicação: ELISA
Espécie: Pan-species (General)
Sensibilidade: The minimum detectable dose of this kit is typically less than 13pg/mL
Duração do ensaio: 2h
Método: Competitive Inhibition
Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique.BSA conjugated VB7 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between BSA conjugated VB7 and unlabeled VB7 (Standards or samples) with HRP conjugated Streptavidin. After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of VB7 in the sample. After addition of the substrate solution, the intensity of color